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Mass spectrometry based lipidomics methodology

Our services

Molecular quantification and characterization

Analysis of fatty acids and sterols.
High sensitivity quantification of groups
of metabolic intermediaries.
Lipidomics: analysis of phospholipids, triglycerides, cholesterol esters and other level intermediates molecular.
Analysis of lipid composition at group level (esterified cholesterol, free cholesterol, acids free fats, phospholipids, sphingolipids).
Sugar analysis.
unit portfolio
introduction to lipidomics oscar pastor rojo
ils 20020 bohdan babiy
Are you interested in our analysis?

These are the steps we follow

Normalization

Type of sample
Sample quantity
Pretreatment, preparation and sample storage 

Extraction

Adequate extraction method
Internal standards to include

Monitoring of lipid ions

Adequate monitoring system:
LC-MS / GC-MS / MALDI-TOF

Process quality control and reproducibility

Evaluation of inter-sample repeatability
Evaluation of inter-day repeatability
Quantitative evaluation of results

Statistical treatment of obtained data

Statistical analysis of groups
Search for outliers
Clustering
ANNOVA evaluation
etc.

Interpretation and graphical representation of results

Bar charts
Dot charts
Box and Whisker charts
Radial charts
etc.

*For more information, please consult the materials and methods section of any of our publications. You can check them on the page “Group publications and projects”.

Do you have any samples you’d like to analyze?

If you are working on a project and want to complete your results with the lipidomic data, need our advice, analytical support or want to collaborate on one of our active projects, do not hesitate to contact us

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