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Unit for Molecular Quantification and Caracterization (UCA-CCM)

Instituto Ramón y Cajal de investigación sanitaria

The Central Support Unit for Molecular Quantification and Characterization (UCA-CCM) was created in 2014 thanks to a public call for infrastructures from the Carlos III Health Institute for accredited Health Research Institutes. The mission of the unit is to provide advice and analytical support to all members of IRYCIS and its Hospital in research and assistance activities that require the application of chromatographic techniques (gas chromatography or liquid chromatography), in combination with traditional detectors (UV/Vis, fluorimetry, electrochemistry, radioactivity), or in combination with methods of detection by mass spectrometry (triple quadrupole, single quadrupole or MALDI-TOF). The unit has a portfolio of services specialized in lipidomics.

Our goals are:

  1. To develop robust quantitative strategies that allow the massive quantification of molecular species of lipids (lipidomic) in different samples.
  2. Apply these methodologies to the study of lipid metabolism.
  3. To transfer methodological and metabolic knowledge related to lipids to the resolution of clinical problems.
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Óscar Pastor Rojo, PhD
Principal investigator

Specialist in mass spectrometry based lipidomics.
Clinical Biochemistry service facultative at the Ramón y Cajal Hospital in Madrid.

What is lipidomics?

Since the appearance of the term genomics in the mid-1980s, a number of different “omics”  have been emerging in the field of biology. The metabolomics is one of them, and tries to quantify and characterize all the metabolites present in a biological system, as well as their interrelation with their regulatory factors (proteins, RNAs, genes). The extreme variety of metabolites present in a cell or tissue makes it impossible to approach all metabolites quantitatively with a single methodological approach and for this reason lipidomics can be defined as a branch of the metabolomics specialised in the study of lipids. Like all omics, the lipidomics avoids a reductionist vision when studying biological phenomena. In other words, in contrast to studies in which a prior hypothesis is put forward on the involvement of a reduced number of variables, in this case lipids, in a process (studies of directed hypotheses, also known as “targeted”), the capacity of the lipidomics to quantify a high number of lipids makes it possible to understand integrally the functioning of the biological processes studied and the generation of new working hypotheses.

“The molecular characterization of lipids in a biological system, as well as their function and study their relationship with the expression of enzymes and proteins involved in their metabolism and gene regulation.”

lipid diversity

 (Spener, F. y col. Editorial: What is lipidomics (Eur. J. Lipid Sci. Technol. (2003))

introduction to lipidomics oscar pastor rojo
What areas of research do we work in?
Falsk de Thermo Fisher Scientific
Study with cellular models in vitro
Experimentación animal
Models with experimental animals
Enfermedad de Gaucher
Translation of the results obtained to the diagnosis and monitoring of diseases

Lipidomics research achievements

Participation in the interlaboratory study of lipidomic standardization of human plasma
Development of preclinical models of nonalcoholic fatty liver disease
Synthesis of new DEGS1 enzyme inhibitors
Methodological development for rapid quantification of lipid profile in patient plasma
Analysis of biomarkers of Gaucher disease
New approaches to lipidomics quantification and its application in multiple sclerosis
ils 2021 oscar pastor rojo
joined 2022 bohdan babiy
ils 20020 bohdan babiy
metodología lipidómica omega 3 silvia recuero
lipidómica en la esclerosis múltiple jorge serna
Our portfolio of services
Basic lipid extraction
Sphingolipid extraction
Standard lipidomics (CE, TG, PE, PC, DG, LPC, LPE, SM)
Sphingolipids lipidomics (SM, Cer, dhCer, HexCer, LacCer, Sulf)
Acid phospholipid lipidomics (PI,PS,CL,PG,BMP)
Bioinformatic analysis 
Our group in the press
Un polifenol presente en muchas frutas y frutos secos ejerce un efecto neuroprotector frente a la esclerosis múltiple en un modelo animal
Un polifenol presente en muchas frutas y frutos secos ejerce un efecto neuroprotector frente a la esclerosis múltiple en un modelo animal
Demuestran que la curcumina ayuda a que las células eliminen lípidos de desecho
Demuestran que la curcumina ayuda a que las células eliminen lípidos de desecho
joined 2022
Premio en la I Jornada de Investigación de Estudiantes de Doctorado del IRYCIS
Do you want to apply lipidomics to your projects?

If you are working on a project and want to complete your results with the lipidomic data, need our advice, analytical support or want to collaborate on one of our active projects, do not hesitate to contact us.

Do you want to train in the field of lipidomics?

If you are interested in starting your career in the field of metabolomics, learning how to operate mass spectrometry equipment and perform lipidomics, make a stay in our laboratories as an assistant, develop your final project / master’s degree / thesis with us, do not hesitate to contact us. 

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